The summer blockbuster show will be one of the 95-year-old artist's most comprehensive exhibitions ever staged globally, and her largest in Australia.
The ticketed show will span eight decades of Kusama's art, from her childhood in the 1930s, to her years at the forefront of the New York art scene, and her current work.
Opening in December, it will include 180 works and the unveiling of her most recent immersive infinity mirror room, which is currently being developed for the show.
The world premiere exhibition includes painting, sculpture, collage, fashion, video and installation and will take up the entire ground floor of the NGV International building.
As visitors enter the gallery, they will first encounter her installation Narcissus Garden from the 1960s, which is made of more than 1400 stainless silver balls, each reflecting the person looking.
Narcissus Garden is made up of more than 1400 mirrored balls. (EPA PHOTO)
The exhibition also includes a new NGV acquisition, the five metre tall Dancing Pumpkin sculpture, which gallery-goers can walk under.
Another installation on show in Australia for the first time, THE HOPE OF THE POLKA DOTS BURIED IN INFINITY WILL ETERNALLY COVER THE UNIVERSE, is made from six-metre-high tentacles covered in her iconic yellow and black patterns.
Kusama was born in Japan in 1929 and has contributed not only to pop art but minimalism and feminist art over almost a century.
Yayoi Kusama opens December 15 at the National Gallery of Victoria.
