The 53-metre-tall, three-stage launcher left the launch pad in the French spaceport of Kourou on its 117th and final mission at 7pm local time (2300 GMT) on Wednesday, deploying two satellites on schedule roughly 30 minutes later, according to a live webcast.
"Ariane 5 is now over, and Ariane 5 has perfectly finished its work," Arianespace CEO Stephane Israel said on the webcast.
The mission to send France's Syracuse 4B and Germany's Heinrich Hertz (H2Sat) satellites to geostationary orbit caps 27 years of service for Ariane 5, whose successor - Ariane 6 - has been hit by technical delays until 2024 for operational use.
Europe until recently depended on Ariane 5 and its 11-tonne-plus capacity for heavy missions, as well as Russia's Soyuz launcher for medium payloads and Italy's Vega for small ones.
But Moscow last year withdrew access to Soyuz amid tensions over Ukraine and the upgraded Vega C remains grounded after the failure of its second launch in December, sparking what the head of the European Space Agency has termed a space launch "crisis".
The CEO of Airbus, which co-owns manufacturer ArianeGroup with France's Safran, said in June the gap highlighted Europe's "vulnerability" in space.Â
"All pressure is now on Ariane 6," Guillaume Faury told the Paris Air Forum.
The first test launch of Ariane 6 is expected at the end of the year depending on tests to be carried out in the summer, with the first commercial operation planned for next year.
The final launch of Ariane 5 was delayed last month for technical reasons and again this week because of weather.
Led initially by France, Germany and the UK, Europe's Ariane series pioneered commercial launches but now faces intense competition from Elon Musk's SpaceX, prompting the development of a more inexpensive Ariane 6 to better compete with the Falcon 9.
Development of the family of launchers began in 1973, with the first Ariane 1 lifting off in 1979.
Ariane 5's maiden flight in 1996 ended in failure as the rocket went off course after 40 seconds and self-destructed.
But the new workhorse took part in several launch milestones including the James Webb Telescope in 2021, in partnership with NASA and the Canadian Space Agency, and Europe's comet-chasing Rosetta mission, which deployed a landing probe in 2014.
