"As part of its terrorist activities, Hezbollah is using ambulances extensively for military purposes," the Israeli military's Arabic spokesman Avichai Adraee said on X, adding that such use must immediately stop.
"If this practice does not stop, Israel will act in accordance with international law against any military activity carried out by the terrorist group Hezbollah using these facilities and ambulances," Adraee said.
A Hezbollah official said that the group was not using ambulances and medical facilities for military purposes.
The Israeli military did not immediately respond to a request to provide evidence that Hezbollah was using medical facilities or ambulances unlawfully.
At least 26 medics and first responders have been killed in Israeli strikes since March 2 according to the Lebanese Health Ministry.
The Israeli military says it takes precautions to try to reduce any harm to civilians.
Iran-aligned Hezbollah opened fire at Israel on March 2, saying it was retaliating for the killing of Iran's supreme leader at the start of the US-Israeli war on Iran.
Israel has since launched an extensive bombing campaign against the powerful Lebanese armed group, which has killed more than 700 people and displaced hundreds of thousands more, while Hezbollah has fired hundreds of rockets across the border.
On Friday, Israeli aircraft dropped flyers over Beirut threatening to inflict damage on Lebanon similar to the devastation wrought on the Gaza Strip during Israel's two-year war with Palestinian militant group Hamas.
Much of the Gaza Strip has been reduced to rubble and its population largely displaced.
An Israeli official told Reuters on Friday that the campaign against Hezbollah would likely be intensified and continue even after strikes on Iran die down.
The official said that attacks on civilian infrastructure were being debated by the decision-makers.
Hezbollah leader Naim Qassem said on Friday that his group was prepared for a long confrontation.
Israeli newspaper Haaretz reported on Saturday that Israel and Lebanon were expected to hold direct talks in the coming days, citing two sources with knowledge of the matter.
US President Donald Trump's son-in-law Jared Kushner will be involved in the talks that may be held in Paris or in Cyprus, with Israeli Prime Minister Benjamin Netanyahu's confidant Ron Dermer leading the Israeli delegation, Haaretz said.
The negotiations were expected to focus on ending fighting in Lebanon and disarming the Lebanese armed group Hezbollah, Haaretz said.